Journal: Chemistry & Biodiversity

Article Title: Network Pharmacology and In Vivo Evaluation of Modified Huanglian Wendan Decoction in Polycystic Ovary Syndrome With Insulin Resistance: Association With AGE–RAGE‐Related Signaling

doi: 10.1002/cbdv.71737

Figure Lengend Snippet: Network pharmacology analysis of HLWDD for PCOS. (A) Representative hub‐gene protein‐protein interaction subnetwork derived from the overlapping target set. (B) Gene Ontology enrichment analysis. (C) KEGG pathway enrichment analysis, including AGE–RAGE signaling in diabetic complications among the enriched pathways.

Article Snippet: Primary antibodies included AGE (A16422, ABclonal; 1:2000), RAGE (66833‐1‐Ig, Proteintech; 1:2000), STAT3 (60199‐1‐Ig, Proteintech; 1:1000), AKT1 (10176‐2‐AP, Proteintech; 1:2000), phospho‐STAT3 (Ser727; AP0715, ABclonal; 1:2000), phospho‐AKT (Ser473; 80455‐1‐RR, Proteintech; 1:1000), and GAPDH (60004‐1‐Ig, Proteintech; 1:20000).

Techniques: Analysis, Derivative Assay

Journal: Chemistry & Biodiversity

Article Title: Network Pharmacology and In Vivo Evaluation of Modified Huanglian Wendan Decoction in Polycystic Ovary Syndrome With Insulin Resistance: Association With AGE–RAGE‐Related Signaling

doi: 10.1002/cbdv.71737

Figure Lengend Snippet: Ovarian AGE–RAGE/STAT3/AKT1‐related protein expression and phosphorylation. (A) Representative Western blot bands for AGE, RAGE, STAT3, AKT1, phospho‐STAT3 (Ser727), phospho‐AKT (Ser473), and GAPDH in the Control, Model, HLWDD‐L, HLWDD‐M, HLWDD‐H, and Metformin groups. (B) Relative densitometric analysis of AGE/GAPDH, RAGE/GAPDH, STAT3/GAPDH, AKT1/GAPDH, p‐STAT3/STAT3, and p‐AKT/AKT1. ** p < 0.01 vs. Control; ## p < 0.01 vs. Model. Densitometric comparisons were based on independent biological replicates and analyzed as described in Methods.

Article Snippet: Primary antibodies included AGE (A16422, ABclonal; 1:2000), RAGE (66833‐1‐Ig, Proteintech; 1:2000), STAT3 (60199‐1‐Ig, Proteintech; 1:1000), AKT1 (10176‐2‐AP, Proteintech; 1:2000), phospho‐STAT3 (Ser727; AP0715, ABclonal; 1:2000), phospho‐AKT (Ser473; 80455‐1‐RR, Proteintech; 1:1000), and GAPDH (60004‐1‐Ig, Proteintech; 1:20000).

Techniques: Expressing, Phospho-proteomics, Western Blot, Control, Analysis

Journal: Chemistry & Biodiversity

Article Title: Network Pharmacology and In Vivo Evaluation of Modified Huanglian Wendan Decoction in Polycystic Ovary Syndrome With Insulin Resistance: Association With AGE–RAGE‐Related Signaling

doi: 10.1002/cbdv.71737

Figure Lengend Snippet: Working model integrating the computational and in vivo findings. Network pharmacology and docking prioritized AGE–RAGE/STAT3/AKT1‐related signaling as a candidate network; 100‐ns MD and MM/PBSA analyses supported the dynamic plausibility of three representative docked complexes. In vivo treatment was associated with reproductive, insulin‐related metabolic (including HOMA‐IR), inflammatory, total‐protein, and phosphorylation‐level changes. Dashed connectors denote association; direct target engagement and causal pathway mediation were not established.

Article Snippet: Primary antibodies included AGE (A16422, ABclonal; 1:2000), RAGE (66833‐1‐Ig, Proteintech; 1:2000), STAT3 (60199‐1‐Ig, Proteintech; 1:1000), AKT1 (10176‐2‐AP, Proteintech; 1:2000), phospho‐STAT3 (Ser727; AP0715, ABclonal; 1:2000), phospho‐AKT (Ser473; 80455‐1‐RR, Proteintech; 1:1000), and GAPDH (60004‐1‐Ig, Proteintech; 1:20000).

Techniques: In Vivo, Docking Assay, Phospho-proteomics, Drug discovery